There are men's and women's restrooms available in the lobby of the Constitution Ave entrance.
The first floor has restrooms available in two different locations:
- Ocean Hall – off the museum’s central hall (to the right) there are restrooms for men, women, and families (a companion restroom) available on this side of the museum.
- African Voices - there are men's and women's restrooms at the end of the exhibit behind the Freedom Theater. (Insiders tip: there are rarely lines here!)
- IMAX® Theater - there are men's and women's restrooms near corridor between the Ocean Hall and the IMAX® Theater.
- Human Origins Hall - there are men's and women's restrooms available near the middle of the exhibit.
Located at the very end of the temporary exhibit Beyond Bollywood, near the exhibit case Backyard Dinosaurs and the FossiLab, there is access to men's and women's restrooms.