Table of Contents for Protocols

The workshop protocols are typically used in the following order if you are proceeding from tissue samples to DNA sequences (download):

1. Course overview
2. DNA extraction
3. DNA concentrations with gel
4. PCR
5. Gel electrophoresis
6. PCR product purification
7. Cycle sequencing reaction
8. Centri-sep column purification (or alternatively, Sephardex slurry protocol)
9. Sequence product prep
10. 310 assembly and sequencing
11. 310 breakdown
12. Sequence contigs and alignment
13. Stock solutions protocol is used throughout, especially with PCR